Fig. 3: Regulation of various genes by split ON switch system.

a, c, e Schematic illustration of split ON switch system regulating three antibiotic resistance genes: puromycin N-acetyltransferase (PAC) (a), hygromycin-B 4-O-kinase (HPH) (b), and blasticidin-S deaminase (BSR) (c). The experimental time course is shown below each schematic illustration. b, d, f The survival rate of HeLa cells treated with miR-21-5p or NC inhibitors as measured by the WST-1 assay. For each condition, the survival rates were calculated by dividing the viability in the presence of antibiotics by that without antibiotics. Error bars represent means ± SD (n = 3), and data of each biological replicate are shown as a point. g Schematic illustration of split ON switch system regulating herpes simplex virus type 1 thymidine kinase (HSV-TK). h The survival rate of HeLa cells treated with miR-21-5p or NC inhibitors as measured by the WST-1 assay. For each condition, the survival rates were calculated by dividing the viability in the presence of ganciclovir (GCV) by that without GCV. Error bars represent means ± SD (n = 3), and data of each biological replicate are shown as a point. Statistical analysis by two-sided Welch’s ttest (b, d, f, h), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s.: not significant (P > 0.05). Each P-value is listed in Supplementary Table 4. Source data are provided as a Source Data file.