Fig. 4: Glycan binding studies of wt and Δfap2 Fusobacterium nucleatum with sequence-defined covalent microarrays reveals binding to sialylated, fucosylated and galactose-terminating glycans. | Nature Communications

Fig. 4: Glycan binding studies of wt and Δfap2 Fusobacterium nucleatum with sequence-defined covalent microarrays reveals binding to sialylated, fucosylated and galactose-terminating glycans.

From: Identification and characterisation of vaginal bacteria-glycan interactions implicated in reproductive tract health and pregnancy outcomes

Fig. 4

(Left panels) Heatmaps of relative binding strengths of fixed fluorescently labelled F. nucleatum 23726 to neutral and sialylated glycans at pH7 in the presence of 10 mM galactose, 10 mM glucose or buffer only. Source data are provided in Source Data File 5. (Right panels) Heatmaps of relative binding strengths of fixed fluorescently labelled wt, Δgalkt and Δgalkt Δfap2 F. nucleatum 23726 to neutral and sialylated glycans at pH7. Values on heatmap indicate the mean fluorescence intensities of quadruplicate spots on Microarray set 2a and 2b. Colour code for binding intensities: Dark blue (0–10%); Light blue (10–25%); Yellow (25–50%); Orange (50–70%); Red (70–100%). 100%, the maximum binding score observed across the three conditions tested. Glycan structures corresponding to selected bound glycan probes are shown. Glycans categories are coloured as follows: Yellow: Mucin core related; Pink: Sialylated; Orange: LacNAc terminating; Blue: Blood group A, B, H glycans; Peach: Lewis blood group related; Purple: Fucosylated branched glycans from human milk; Grey: Non-sulphated chondroitin sulphate. * (-104) Grey cells were flagged for artefact/background on slide.

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