Fig. 5: Glycosaminoglycan binding by bacteria of the vaginal microbiota. | Nature Communications

Fig. 5: Glycosaminoglycan binding by bacteria of the vaginal microbiota.

From: Identification and characterisation of vaginal bacteria-glycan interactions implicated in reproductive tract health and pregnancy outcomes

Fig. 5

A Heatmap showing the relative binding intensities to 36 GAG oligosaccharides at pH4 and pH7 of fixed fluorescently labelled strains of E. coli and F. nucleatum, and live L. crispatus, L. iners, G. vaginalis and S. agalactiae. The average rank of the mean fluorescence intensities from at least two independent experiments on Microarray set 5 was calculated (Source Data file 6) and the resulting values coloured as follows: Dark blue (0–10 %); Light blue (10–25 %); Yellow (25–50 %); Orange (50–70%); Red (70–100%). 100%, the maximum binding score observed across pH4 and pH7 for a given strain. B Glycan microarray images of fluorescently labelled E. coli C600, F. nucleatum 23726, L. crispatus 1398, L. iners 13335, G. vaginalis 775 and S. agalactiae 776 binding to GAG polysaccharides at pH4 or pH7. Images are representative of three independent experiments. C Bar charts showing the mean fluorescence intensities of bacterial binding at pH4 or pH7 to GAG polysaccharides on Microarray set 6 printed at 0.5 mg/ml. Error bars represent SD of quadruplicate spots for each polysaccharide on the microarray (n = 1). Assay is representative of three independent experiments. Source data are provided in Source Data File 7.

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