Fig. 6: Analysis of bacterial binding to VK2 vaginal epithelial cells and glycan binding competition by bacteria on glycan microarrays. | Nature Communications

Fig. 6: Analysis of bacterial binding to VK2 vaginal epithelial cells and glycan binding competition by bacteria on glycan microarrays.

From: Identification and characterisation of vaginal bacteria-glycan interactions implicated in reproductive tract health and pregnancy outcomes

Fig. 6

A Flow cytometry-based analysis of live bacterial binding to VK2 cells treated or not with chondroitinase ABC. Bar charts show the mean fluorescence intensities of the bound bacteria at pH4 and pH7. Error bars represent SD of three independent experiments for each condition (n = 3). B Flow cytometry-based analysis of the binding of L. crispatus preincubated or not with CSC polysaccharide to VK2 cells. Bar charts show the mean fluorescence intensities of the bound bacteria at pH4 and pH7. Error bars represent SD of three independent experiments for each condition (n = 3). C Left: Bar charts show the mean fluorescence intensities of the binding at pH4 of fluorescently labelled S. agalactiae 776 and L. crispatus 1398 to a CSC 14-mer oligosaccharide in the presence of increasing amounts of L. crispatus 1398. Error bars represent SD of triplicates on the microarray for each glycan (n = 1). One experiment representative of two independent experiments is shown. Right: Representative images of S. agalactiae 776 cells binding to CSC 14-mer on the microarray in the presence of increasing amounts of L. crispatus 1398. *** p < 0.0001; *p < 0.039; ns: non-significant; 2-way ANOVA (A) and **p < 0.0067; ns: non-significant B). Source data are provided in the Source Data File 7.

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