Fig. 4: In vivo release of model cargoes loaded within PPSU microgels.
From: A biomimetic multi-component subunit vaccine via ratiometric loading of hierarchical hydrogels
a Real-time whole-body imaging of adsorbed ICG after SC injection showed prolonged release of hydrogel cargo. The data are presented as mean values with the statistical significance determined by a two-sample t-test (n = 3 mice per group). b Efficient FRET demonstrates full accessibility (within ~10 nm) of the microgel surfaces by small molecule fluorophore Rh101. c The recovery of Rh6G fluorescence at day 1 indicated quick desorption/replacement of adsorbed Rh101 upon SC injection. λem/λex = 465/640 nm for Rh101, λem/λex = 465/560 nm for Rh6G. The data are presented as mean values with the statistical significance determined by a two-sample t-test (n = 5 mice per group). d Not all of the microgel surfaces are accessible to proteins, as suggested by the inefficient FRET from encapsulated MFT in response to incubation with TNF-α. e Sustained release was achieved for the encapsulated MFT and adsorbed proteins. λem/λex = 465/600 nm for TNF-α, λem/λex = 465/520 nm for MFT. The data are presented as mean values with n = 3 mice per group.
