Fig. 3: Advanced SGC cluster into 3 groups of immune infiltration.

A Heatmap of GSVA scores of 6 immune signatures (see methods) for all samples (n = 95). Samples were clustered by hierarchical clustering and annotated by tumor entity (ACC/non-ACC or 9 categories of tumor entities), cell-of-origin group (ID/ED), sample type (metastasis/primary) and prior systemic therapy status. B GSVA scores of 6 immune signatures were calculated in an integrated analysis together with previously published cohorts (n = 198). Samples were annotated by tumor entity (ACC/non-ACC), sample type (metastasis/primary) and cohort (MASTER cohort/Linxweiler et al/Vos et al). C Comparison of immune clusters (Immune-high n = 33 median = 0.48, iqr = 0.29, max = 0.88, min = −0.13; Immune-medium n = 34 median = −0.38, iqr = 0.24, max = 0.28, min = −0.62; Immune-low n = 28 median = −0.67, iqr=0.19, max = −0.38, min = −0.89) to TCGA most (PAAD n = 183 median = −0.08, iqr = 0.87, max = 0.94, min = −0.83; TGCT n = 156 median = 0.40, iqr = 0.69, max = 0.88, min = −0.76; LUSC n = 553 median = 0.47, iqr = 0.68, max = 0.97, min = −0.91; HNSC n = 566 median = 0.56, iqr = 0.79, max = 0.98, min = −0.87; LUAD n = 600 median = 0.55, iqr = 0.52, max = 0.97, min = −0.80) and least inflamed cohorts (LGG n = 534 median = −0.82, iqr = 0.18, max = 0.69, min = −0.96; PCPG n = 187 median = −0.63, iqr = 0.24, max = 0.62, min = −0.88; ACC n = 79 median = −0.63, iqr = 0.28, max = 0.77, min = −0.84; UVM n = 80 median = −0.68, iqr = 0.34, max = 0.85, min = −0.93; KICH n = 91 median = −0.47, iqr = 0.41, max = 0.38, min = −0.79). D Intensity of pan T-cell marker (CD3) versus IFNG bulk score. Samples were colored by immune clusters (n = 14, Immune-high n = 6 median=0.83, iqr=0.13, max=0.85, min=0.26; Immune-medium n = 3 median = −0.17, iqr=0.71, max=0.89, min = −0.84; Immune-low n = 5 median = −0.73, iqr=0.06, max = −0.67, min = −0.80). E One-way anova test was performed to analyze the association of several clinical parameters with immune scores (APM and IFNG). Corrected and log-transformed p-values are provided in the heatmap, showing a significant impact of tumor entity, cell-of-origin, tumor purity, and TMB.