Fig. 3: Comprehensive analysis of mouse N-glycoproteome. | Nature Communications

Fig. 3: Comprehensive analysis of mouse N-glycoproteome.

From: Ultradeep N-glycoproteome atlas of mouse reveals spatiotemporal signatures of brain aging and neurodegenerative diseases

Fig. 3

a Distribution of molecular masses for identified N-glycopeptides, illustrating the contributions from peptide and glycan components. b Comparison of theoretical and experimental molecular mass distributions of de-glycosylated N-glycopeptides. c Distribution of glycan composition mass across different mouse tissues. d Pie chart showing the distribution of secondary structure elements (coil, bend, hydrogen-bonded turn, extended strand in β ladder, 3-helix, α-helix, 5-helix, β-bridge) as predicted by AlphaFold2 and annotated by DSSP. e Three-dimensional histogram illustrating the distribution of glycoproteins by the number of glycosites and glycoforms per protein. f Scatter plot showing the distribution of glycosites and glycoforms per protein across different tissues. g Venn diagram and radar chart showing the distribution of glycosites and glycoforms of the CD36 protein across different tissues. h Western blot analysis of CD36 glycosylation in mouse tissues. Samples from heart, liver, brain, lung, and kidney were analyzed with (+) or without (−) PNGase F treatment. The experiments were repeated in four technical replicates with similar results.

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