Fig. 5: IL17A promotes tuft cell differentiation and asthmatic airway epithelium contains mature tuft cells. | Nature Communications

Fig. 5: IL17A promotes tuft cell differentiation and asthmatic airway epithelium contains mature tuft cells.

From: Single cell profiling of human airway identifies tuft-ionocyte progenitor cells displaying cytokine-dependent differentiation bias in vitro

Fig. 5

A–D Tuft cell abundance increases following IL17A treatment in LAE ALI. A, C Overview schematic of IL17A treatment experiments in mature LAE ALI treated with PBS (control) or IL17A (50 ng/ml; from D39 to D44) (A) and differentiating ALI at D3 (the first time point at which TIP cells are present) treated cells with IL17A (50 ng/ml) or PBS from D3 to D24 (C). B Number of GNAT3+ mature tuft cells (quantified by immunohistochemistry, y axis, n = 2 ALIs (Hu19, Hu67)), when mature LAE ALIs are treated with PBS or IL17A (50 ng/ul; from D39 to D44) (x axis) D Number of GNAT3+ mature tuft cells (quantified by immunohistochemistry, y axis, n = 2 ALIs, (Hu19, Hu67)), in LAE ALIs when treated with PBS or IL17A from D3 to D24. E, F Tuft cells are increased in ALIs and airways from asthmatic patients. E Number of GNAT3+ mature tuft cells (y axis) in LAE ALIs derived from two asthmatic individuals (Hu70 and Hu78) and in patients with no history of lung diseases (Hu66, Hu67) (x axis), n = 1 ALI per donor. F Whole mount staining of dissected airways from a patient with asthma exacerbation. Staining in the top panel with GNAT3 (green) shows a mature tuft cell, surrounded by ciliated cells (Atub, white). Staining with additional mature tuft cell marker ALOX5AP (magenta) reveals the characteristic bipolar morphology (arrows) associated with mature tuft cells (bottom panels). Due to the availability of tissue, immunostaining was performed on only 1 donor. Illustrations 4B and D were created with BioRender: https://BioRender.com/g37wh81.

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