Fig. 3: O-MRDE/Mn was efficiently taken up, inducing ICD and activating immune responses. | Nature Communications

Fig. 3: O-MRDE/Mn was efficiently taken up, inducing ICD and activating immune responses.

From: Neoantigens combined with in situ cancer vaccination induce personalized immunity and reshape the tumor microenvironment

Fig. 3

a Illustrative diagram for the function of each component. b Investigating the mechanism of tumor cellular uptake of O-MRDE/Mn. c Pearson’s correlation coefficient analysis of lysosome escape of D4F-DE/Mn, MDE/Mn, and O-MRDE/Mn. d Cytotoxicity of O-MRDE/Mn+CXCL10@Gel against GL261 cells. e Flow cytometry was used to detect CRT on GL261 cells. f, g Levels of HMGB1 (f) and ATP (g). h, i Expression of CD80, CD86 (h), and CD40 (i) on BMDCs. j Levels of antigen SIINFEKL-presented BMDCs. k Quantitative results of coculture-induced CD8+ T cells activation. l Quantitative results of coculture-induced SIINFEKL-specific CD8+ T cells activation. m Quantitative analysis of the levels of different cytokines using ELISA. n Schematic diagram of innate immune activation of O-MRDE/Mn. o, p Content of cGAMP (o) and IFN-β (p). Data in b–h, j–l, m, o, and (p) were mean ± SD (n  =  3 independent experiments). The significance between each of the multiple groups in b, e–h, j–l, o, and (p) was calculated using one-way ANOVA. Source data are provided as a Source Data file.

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