Fig. 4: O-MRDE/Mn induces innate and adaptive immune responses in vivo. | Nature Communications

Fig. 4: O-MRDE/Mn induces innate and adaptive immune responses in vivo.

From: Neoantigens combined with in situ cancer vaccination induce personalized immunity and reshape the tumor microenvironment

Fig. 4

a Diagram illustrating the experimental setup. b Images of tumor tissues stained with H&E. c Bioluminescence imaging of mice bearing GL261-luc and GL261-OVA-luc tumors. d Quantification of bioluminescent signal intensity. e Survival of mice bearing GL261-luc and GL261-OVA-luc tumors. f Quantitative analysis of cytokine levels using ELISA. g Body weight changes of mice bearing GL261-luc and GL261-OVA-luc tumors. h, i Quantitative analysis of NK cells (CD3-NK1.1+) (h) and mature DCs (CD11c+MHC I+) (i) in tumor. j–l Quantification of CD8+ T cells (CD3+CD8+) (j), IFN-γ+ T cells (CD8+IFN-γ+) (k), and GrB+ T cells (CD8+GrB+) (l) in spleen. m Quantitative analysis of IgM, IgG, and IgA levels using ELISA. Data were mean ± SD (n = 6 mice per group). The significance in (d) between G1 and G2-G7, and between G8 and G9 was calculated using two-way ANOVA. The significance in (e) between G1 and G2–G7, and between G8 and G9 was calculated using Kaplan-Meier survival analysis. The significance between each of the multiple groups in (h–m) was calculated using one-way ANOVA. Source data are provided as a Source Data file.

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