Fig. 4: Early transcriptional response to depletion of RNases P and MRP.
From: Reversible proliferative arrest induced by rapid depletion of RNase MRP
A Impact of dTAG on steady-state mRNA levels. Volcano plots showing differential mRNA abundances between C40 cells treated with DMSO or dTAG for 3 h (RNA-seq data; n = 3). Gray dots indicate all evaluated genes, and colored dots genes belonging to the indicated functional categories (see also Supplementary Fig. 4B and Supplementary Data 1). Colored numbers above the volcano plots are counts of significantly regulated color-highlighted genes (adjusted p-value (padj) < 0.01) in different bins separated by dashed vertical lines according to the strength and sense of regulation. Insets summarize the total numbers of significantly up- or downregulated color-highlighted genes. FC, fold change. See also Supplementary Fig. 4 for changes at 24 h of depletion. B As in (A), showing the impact of dTAG treatment for 3 h on nascent transcripts (TTchem-seq data; n = 3). C Correlation of RNA-seq and TTchem-seq data at 3 h (left) and 24 h (right) of treatment. Red dots indicate significantly regulated histone genes (padj < 0.01). Differentially expressed genes in (A, B) were identified using the DESeq2 package. P-values were adjusted for multiple testing using the Benjamini-Hochberg method to control the false discovery rate.
