Fig. 7: Binding assay and EMPEM. | Nature Communications

Fig. 7: Binding assay and EMPEM.

From: Antigen persistence and TLR stimulation contribute to induction of a durable HIV-1-specific neutralizing antibody response

Fig. 7

A Sera from week 28 from rabbits (n = 6) immunized with the indicated vaccine regimen was assayed against the indicated stabilized trimer protein. The area under the curve (AUC) of serial dilutions is displayed. B Negative-stain EMPEM 3D reconstruction of polyclonal antibody fragment antigen-binding (pFab) from rabbit 142 (RB142) at week 31 in complex with 1086 C NFL trimer (left). The map is segmented and colored by component. Docking of an Env gp140 protomer model into the map reveals that the polyclonal antibodies bind near the V3 and V1 regions of gp120, near N301 (middle and right panels). Relevant potential N-linked glycosylation sites in 1086 C NFL are labeled in green, and those commonly present in other HIV-1 genotypes but not in 1086 C are labeled in red.

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