Fig. 4: Serotonin signaling is not required for lymph gland hematopoiesis in response to immune stress but ensures appropriate lamellocyte release into the hemolymph.
From: Serotonergic neurons regulate the Drosophila vascular niche to control immune stress hematopoiesis
A, B H3P (red) detection 6H post parasitism in control (A) and in HandD > 5-HT1B-RNAi (B) lymph glands. C H3P index in lymph gland anterior lobes. D, E, G, H Lamellocyte differentiation (β-integrin, green) in control (D, G), HandD > 5-HT1B-RNAi (E), and Gyc89Da>Trh-RNAi lymph glands (H). F, I Lamellocyte index in lymph gland anterior lobes. A–I At least three independent experiments were performed, and one is shown. J, K Quantification (%) of circulating lamellocytes upon lymph gland rupture in control (14H post-parasitism) and HandD > 5-HT1B-RNAi (12H post-parasitism) (J) and 21H post parasitism (K). The mean of two (J) and three (K) independent experiments is represented: control n = 7 (J) and 29 (K); 5-HT1B-RNAi n = 16 (J) and 30 (K). Error bars represent SEM. Statistical test: Mann-Whitney nonparametric test, two -tailed. * p < 0.05, *** p < 0.001, ns non-significant. For (C, F, I–K) source data are provided as a Source Data file. Scale bars, 20 μm.
