Fig. 3: Sucrose promotes PIF4 accumulation.
a Immunoblots showing the PIF4 levels in 4-d-old Col-0, hmr-5, and phyB-9 seedlings grown under 50 μmol m−2 s−1 R light at either 21 or 27 °C without (-s) or with (+s) sucrose. b Immunoblots showing the PIF4 levels in 4-d-old Col-0 and hmr-22 seedlings under 50 μmol m−2 s−1 R light without or with sucrose treatment during the 21–27 °C transition. c Immunoblots showing the PIF4 levels in 4-d-old Col-0 and sex1-8 seedlings grown under 50 μmol m−2 s−1 R light at either 21 or 27 °C. d Immunoblots showing the PIF4 levels in 4-d-old Col-0 and sex1-8 seedlings grown under 50 μmol m−2 s−1 R light during the 21–27 °C transition. e Quantitative real-time PCR results showing the transcript levels of YUC8, IAA19, and PIF4 in Col-0 and sex1-8 seedlings during the 21–27 °C transition as described in (d). f Immunoblots showing the PIF4 levels in 4-d-old Ler and tps1-12 seedlings grown under 50 μmol m−2 s−1 R light at either 21 or 27 °C. g Immunoblots showing the PIF4 levels in 4-d-old Ler and tps1-12 seedlings grown under 50 μmol m−2 s−1 R light during the 21–27 °C transition. h Quantitative real-time PCR (qRT-PCR) results showing the transcript levels of YUC8, IAA19, and PIF4 in Ler and tps1-12 seedlings during the 21–27 °C transition as described in (g). For (a–d, f, and g), actin was used as the loading control. The relative PIF4 levels normalized to actin are shown. The immunoblot experiments were independently repeated three times with similar results. Error bars for the protein quantifications in (c, d, f, and g) represent the s.d. (n = three biological replicates), and the centers of the error bars indicate the mean. Significant differences between the PIF4 protein levels in Col-0 and the mutants were calculated using two-tailed Student’s t-tests (** p < 0.01, *** p < 0.001); n.s. stands for no significant difference. For (e and h), the transcript levels of the indicated genes were calculated relative to those of PP2A. Error bars represent the s.e. (n = three biological replicates), and the centers of the error bars indicate the mean. Significant differences in the transcript levels of the indicated genes between Col-0 and the mutants were calculated using two-tailed Student’s t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001); n.s. stands for no significant difference. The underlying source data for the immunoblots in (a–d, f, and g) and the qRT-PCR results in (e and h) are provided in the Source Data file.
