Fig. 4: Concerted actions of dual thermosensory pathways trigger PIF4 activity.
a Venn diagram depicting the 173 PIF-dependent genes whose induction requires both the chloroplast-sucrose-mediated and Sensor 2-dependent thermosensory pathways. Sucrose-induced genes: induced genes comparing hmr-5_27S with hmr-5_27; Sensor2-induced genes: induced genes comparing hmr-5_27S with hmr-5_21S; Sensor2-induced PIF-independent genes: induced genes comparing hmr-5pifq_27S with hmr-5pifq_21S. b Heatmap showing the relative expression levels of the 173 dual-sensor-induced and PIF-dependent genes in hmr-5 at 21 and 27 °C with or without sucrose. c GO enrichment analysis of the 173 dual-sensor-induced and PIF-dependent genes. d Heatmap showing the relative expression levels of four auxin biosynthesis genes, YUC2, YUC5, YUC8, and YUC9, in hmr-5 at 21 and 27 °C with or without sucrose treatment. e Images of 4-day-old Col-0, hmr-5, and hmr-5pifq seedlings grown under 50 μmol m−2 s−1 R light at 21 °C on regular growth media or media with sucrose (S), picloram (P), or both sucrose and picloram (S + P). f Hypocotyl length measurements of the Col-0, hmr-5, and hmr-5pifq seedlings described in (e). Error bars represent the s.e. (n = three biological replicates), and the centers of the error bars indicate the mean. Different lowercase letters denote statistically significant differences in hypocotyl length (one-way ANOVA, Tukey’s HSD, p < 0.05, n = 3 biological replicates). The underlying source data for the hypocotyl measurements in (f) are provided in the Source Data file.
