Fig. 6: ROMO1 upregulation reverses aging-associated oxidations of the mitochondrial cysteinome in multiple organs.
From: ROMO1 overexpression protects the mitochondrial cysteinome from oxidations in aging
a–d Distributions of the averaged activity-based protein profiling ratios of Old(WT)/Young(WT) of the mitochondrial cysteinome in the heart (a), skeletal muscle (SKM) (b), liver (c) and brain (d). Blue, gray, and red dots indicate the reduced (Log2R(old/young)≥0.58), unchanged (-0.58<Log2R(old/young)<0.58), and oxidized (Log2R(old/young)≤-0.58) cysteine sites in the old WT mitochondria, respectively. Insets show numbers of cysteine sites being reduced (Re), unchanged (Un), and oxidized (Ox). n = 4 independent experiments from 8 mice for each tissue (4 M 4 F for each group). 3-month-old (young) and 25-month-old (old) WT mice were used. e–h, As in (a–d), except the averaged activity-based protein profiling ratios were Log2R(TGold/WTold). n = 4 independent experiments from 8 mice for each tissue (4 M 4 F for each group). 25-month-old WT and TG mice were used. i–l Venn diagram showing the overlap between the oxidized cysteine sites during aging in WT tissues (Log2R(Old/Young)WT ≤ -0.58) and the reduced cysteine sites by ROMO1 upregulation in aged tissues (Log2R(TG/WT)old ≥ 0.58). Source data are provided as a Source Data file.
