Fig. 7: ROMO1 upregulation retards functional decline of multiple organs during aging.

a, b Echocardiographic analysis of heart functions in WT and TG mice at the age of 3, 15, and 22 months (m). EF, injectional fraction; FS, fractional shortening. Data are mean ± s.e.m. In a, for 3-month old mice, n = 12 (6 M 6 F) in WT and n = 13 (7 M 6 F) in TG; for 15-month old mice, n = 8 (4 M 4 F) in WT and n = 11 (7 M 4 F) in TG; for 22-month old mice, n = 5 (3 M 2 F) in WT and n = 5 (3 M 2 F) in TG. In b, for 3-month old mice, n = 12 (6 M 6 F) in WT and n = 13 (7 M 6 F) in TG; for 15-month old mice, n = 8 (4 M 4 F) in WT and n = 8 (5 M 3 F) in TG; for 22-month old mice, n = 5 (3 M 2 F) in WT and n = 5 (3 M 2 F) in TG. c Relative changes of grip strength of WT and TG male mice at different ages. Data are mean ± s.e.m. For 5-month-old mice, n = 12 in WT and n = 15 in TG; for 9-month-old mice, n = 12 in WT and n = 12 in TG; for 12-month-old mice, n = 10 in WT and n = 11 in TG; for 24-month-old mice, n = 7 in WT and n = 9 in TG. d Attenuated gastrocnemius muscle loss in old TG mice. Data are mean ± s.e.m. For 3-month-old mice, n = 5 in WT and n = 5 in TG; for 25-month-old mice, n = 10 in WT and n = 8 in TG. Male mice were used. BW, body weight; mg, milligram; g, gram. e Cross-sectional area (CSA) analysis of gastrocnemius muscle fiber in 3-month and 25-month-old WT and TG mice. Data are mean ± s.e.m. For 3-month-old mice, n = 3 in WT and n = 3 in TG; for 25-month-old mice, n = 6 in WT and n = 3 in TG. Male mice were used. f–i Analysis of serum biomarkers in young and old WT and TG mice. AST, aspartate aminotransferase; ALT, alanine aminotransferase; LDL, low-density lipoprotein. Data are mean ± s.e.m. In f, n = 8 WT and n = 8 TG 3-month-old mice; n = 12 WT and n = 11 TG 25-month-old mice. In g and h, n = 8 WT and n = 8 TG 3-month-old mice; n = 11 WT and n = 11 TG 25-month-old mice. In i, n = 8 WT and n = 8 TG 3-month-old mice; n = 13 WT and n = 10 TG 25-month-old mice. Male mice were used. j-l, Number of blood monocytes (j) and neutrophils (k) and circulating interleukin-6 (IL-6) level (l) in young and old WT and TG mice. Data are mean ± s.e.m. In j, k, n = 18 WT and n = 18 TG 3-month-old mice; n = 6 WT and n = 10 TG 25-month-old mice. In l, n = 8 WT and n = 8 TG 3-month-old mice; n = 8 WT and n = 7 TG 25-month-old mice. Male mice were used. In a–c, two-tailed unpaired Student’s t-test with Welch’s correction was used; in d–l, two-way ANOVA with Tukey’s multiple comparisons test was used. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.