Fig. 2: Liver cell-type DNA methylation atlas relative to other healthy tissues. | Nature Communications

Fig. 2: Liver cell-type DNA methylation atlas relative to other healthy tissues.

From: Circulating cell-free DNA methylation patterns indicate cellular sources of allograft injury after liver transplant

Fig. 2

a Heatmap of differentially methylated, cell-type-specific blocks (DMBs) identified from reference WGBS data of healthy human cell-types. Each cell in the plot marks the methylation score of one genomic region (rows) at each of 20 cell-types (columns), with up to 50 blocks shown per cell type. The methylation score represents the number of fully unmethylated or methylated read-pairs divided by total coverage for hypo- and hyper-methylated blocks, respectively. b Heatmap highlighting the top 25 hepatocyte-specific DMBs. c Example of one hepatocyte-specific hypomethylated block (highlighted in blue), upstream of ECHS1 highly expressed in hepatocytes (green track). The alignment from the UCSC genome browser depicts the average DNA methylation (DNAm, purple tracks) across WGBS samples from five different liver cell-types as well as PBMC samples. Chromatin organization marks in hepatocytes are displayed (blue tracks) to show accessibility (DNAse I hypersensitivity, DHS) and regulatory function (H3K27ac binding). d Fragment-level visualization of methylation sequencing reads at hepatocyte-specific hypomethylated block in reference WGBS samples from five different liver cell-types.

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