Fig. 1: Metabolic profiling of old HSPCs suggests a low-nutrition state.

a Metabolomic profiling of HSCs and MPP1. b Cluster analysis of metabolites that increased or decreased during HSC aging. The pie chart shows classification of metabolites that decreased during aging. (young, n = 9; middle-aged, n = 3; old, n = 3 biological replicates) c Age-related changes of TCA cycle metabolites in HSCs. Numbers in the chart indicate rates of changes from young to middle-aged and old mice. Gray boxes indicate unmeasured or undetected metabolites (young, n = 9; middle-aged, n = 3; old, n = 3 biological replicates). d Total amounts of TCA cycle metabolites in HSCs or MPP1s from young (Y) and old (O) mice (young, n = 9; old, n = 3 biological replicates). e Levels of ATP and related metabolites in HSCs in various ages (young, n = 9; middle-aged, n = 3; old, n = 3 biological replicates). f Energy charge in HSC (young, n = 9; old, n = 3 biological replicates), ND, not detected; NM, not measured. g–i Total amounts of amino acids (g), protein amounts (h), and total amounts of glycolytic metabolites (i) in HSCs and MPP1s from young (Y) and old (O) mice. (young, n = 9; old, n = 3 biological replicates). Values are mean ± SEM (d–i). d and f–i Two-sided Mann–Whitney U test. e One-way ANOVA with Tukey–Kramer test. *p < 0.05. Source data are provided with this paper.