Fig. 3: Old BM tissue has a metabolic profile indicative of decreased NO signaling. | Nature Communications

Fig. 3: Old BM tissue has a metabolic profile indicative of decreased NO signaling.

From: Decreased non-neurogenic acetylcholine in bone marrow triggers age-related defective stem/progenitor cell homing

Fig. 3

a Procedure for BM tissue metabolomics using an in situ freezing technique. b Classification of metabolites that increased, remained unchanged, or decreased in comparison to young to middle-aged BM (left) or young to old BM (right). The number of metabolites measured is displayed in the center of each chart. c Cluster analysis of metabolites that increased or decreased during aging in HSC and BM. d Ingenuity pathway analysis based on changes in metabolite levels during aging using young mice as a reference. e Cluster analysis of metabolites that increased or decreased in middle-aged or old BM tissues compared to young BM tissue. f Concentrations of NOS and NO signaling-related metabolites in BM tissues of young, middle-aged, and old mice. a–f young, n = 9; middle-aged, n = 8; old, n = 6 biological replicates. g NO concentrations in BM of young and old mice (young, n = 6; old, n = 6 biological replicates). Values are mean ± SEM (f, g). f One-way ANOVA with Tukey–Kramer test. g Two-sided Mann–Whitney U test. *p < 0.05. Source data are provided with this paper.

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