Fig. 3: The FtsZ N-IDR endows the Z-ring with a proper high-level dynamicity.

a Timelapse imaging showing cells expressing indicated FtsZ forms (each fused to mNeonGreen) taken at indicated time points after indicated sites (red boxes) were photobleached; scale bar = 1 μm. b Statistical time-dependent fluorescence recovery curves; the recovery half-times for FtsZ^WT, FtsZ^D10F, and FtsZ^ΔNIDR are 4.48, 7.54, and 13.39 s, respectively. c Live-cell images show the directional motion of indicated FtsZ entities, and kymographs show entities in two cells recorded every 2 s (total = 100 s). Tracked FtsZ condensates are indicated by white arrows. Overall cell shapes were drawn according to brightfield micrographs and are indicated by dotted white lines; scale bar = 0.5 μm. d Statistical treadmill velocities of indicated FtsZ entities are calculated using recorded kymographs and show treadmill velocities for FtsZ^WT, FtsZ^D10F, and FtsZ^ΔNIDR at 42.53 ± 1.886, 28.19 ± 0.9758, and 13.77 ± 0.8098 nm/s, respectively; mean ± SEM; parametric unpaired two-tailed Student’s t-test, ****P < 0.0001; n, entities in individual cells. e Relationship between extent of assembly (K_eq) and rates of assembly (k_on) and disassembly (k_off) for FtsZ protofilaments. f Fluorescence images showing cells expressing indicated FtsZ fused forms and overexpressing MinC; scale bar = 2 μm. g Immunoblot showing photocrosslinked products of indicated FtsZ^K51Bpa forms expressed in cells overexpressing MinC; red asterisks indicate residual crosslinked dimers. Source data of b, d, g are provided in the Source Data file.