Fig. 6: Multi-omics characterization of muscle Rev-erb functions.
From: Muscle Rev-erb controls time-dependent adaptations to chronic exercise in mice
a Venn diagram showing the ZT22 and ZT10 overlap of differentially expressed genes (DEGs) in KO vs. WT as identified by RNA-seq at 6 months of age (q < 0.05, fold change > 1.5). WT are Rev-erbfloxed mice. b, c Top enriched KEGG pathways in DEGs at ZT22 and ZT10. d, e Heat map of DEGs showing the relative abundance (in Z-score) involved in glycolysis, TCA cycle, fatty acid metabolism, BCAA metabolism, circadian clock, muscle contraction, adhesion, sarcomere, and insulin signaling. f Pie chart representing the genomic distribution of Rev-erbα ChIP-seq peaks. g Top enriched motif of Rev-erbα peaks at ZT10. h Venn diagram showing the overlap of Rev-erbα binding peaks at ZT10 and ZT22. i Heat map of Rev-erbα ChIP-seq signals at ZT10 and ZT22. j, k Browser tracks of Rev-erbα peaks at genes involved in metabolism. l Metabolomics heat map showing the relative abundance (in Z-score) of metabolites at the indicated ZT in quadriceps muscle at 10 months of age (n = 3 mice per group). WT are Rev-erbfloxed mice. Statistical differences were determined using 2-way ANOVA followed by the Tukey test and were indicated on the right side by colors as illustrated in the color key.
