Fig. 1: Ap site has distinct effects on Pol I and Pol II elongation. | Nature Communications

Fig. 1: Ap site has distinct effects on Pol I and Pol II elongation.

From: Cryo-EM uncovers a sequential mechanism for RNA polymerase I pausing and stalling at abasic DNA lesions

Fig. 1

a The natural Ap site exists in equilibrium between the closed sugar hemiacetal and the open aldehyde. The synthetic analog tetrahydrofuran (hereafter termed Ap for simplicity) was used in this study as a mimic of the natural Ap site. b Full mismatched bubble scaffold used for in vitro transcription assays. c In vitro RNA extension assay on four different mismatched bubbles. Each lane corresponds to incubation times of 0, 1, 15, and 60 min. d RNA extension assay with the same scaffold as in (a) but using individual NTPs. ‘T’ and ‘S’ state for ‘Time’ and ‘Start (12mer)’. These experiments were repeated twice. Source data are provided as a Source Data file.

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