Fig. 3: Paused Pol I by Ap site at i + 1 with A12-Ct in the funnel. | Nature Communications

Fig. 3: Paused Pol I by Ap site at i + 1 with A12-Ct in the funnel.

From: Cryo-EM uncovers a sequential mechanism for RNA polymerase I pausing and stalling at abasic DNA lesions

Fig. 3

a Bar diagram of subunit A12 and Ap-RCC structure showing the location of A12 structural elements. b Cryo-EM map of the Ap-RCC structure showing density for A12-linker and A12-Ct. The template and non-template strands are in blue and cyan, respectively, while RNA is in red. c Superposition of Ap-RCC (colors) with Closed Ap-PEC (grey). The A12-Ct catalytic acidic loop (residues 103–108) in Ap-RCC was modelled in poor density. The map around Y717 in subunit A135 is shown as surface in both cases. d Superposition of Ap-RCC (colors) with a Pol I pre-initiation complex where A12-Ct occupies the funnel (PDB code 6RUO, dark green) and free monomeric Pol I with A12-Ct in the funnel (PDB code 5M3M, pink). e Superposition of Ap-RCC (yellow) with Closed (purple) and Open (blue) Ap-PEC in the two different views.

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