Fig. 4: Structures of Pol I paused by an Ap site at i + 1 lacking lobe-associated subunits. | Nature Communications

Fig. 4: Structures of Pol I paused by an Ap site at i + 1 lacking lobe-associated subunits.

From: Cryo-EM uncovers a sequential mechanism for RNA polymerase I pausing and stalling at abasic DNA lesions

Fig. 4

a Overall structures of Ap-PEC* and Ap-PEC** with a close-up view around the active center of their superposition with Closed Ap-PEC (grey). b Structural differences around the lobe of Closed Ap-PEC, Ap-PEC*, and Ap-PEC**. Cryo-EM maps around the lobe and associated subunits are shown as transparent surfaces. c Superposition between Ap-PEC* (yellow) and Pol I* with undamaged DNA and GMPCPP (PDB code 6HLQ, dark blue). d Superposition between Ap-PEC* (salmon with A12 in purple) and Ap-RCC (grey with A12 in yellow).

Back to article page