Fig. 3: Nucleotide cycling and dynamic landscape of the E5 helicase.

a Schematic representation of the nucleotide cycling. b Top view of the AAA+ domains of E5-ssDNA-ATP complex (structure 1 and structure 3), colored according to the local resolution. c Tables presenting the identity and occupancy of nucleotides in the nucleotide binding pockets of structure 1 and structure 3. All EM densities of nucleotides are shown at the same isosurface threshold within each dataset. Protomer holding ATP or ADP is defined as cis, and clockwise adjacent protomer is defined as trans.