Fig. 6: NPCs differentiated from distributed 3D NPC cultures mature into functional neurons. | Nature Communications

Fig. 6: NPCs differentiated from distributed 3D NPC cultures mature into functional neurons.

From: Viscoelastic N‑cadherin-like interactions maintain neural progenitor cell stemness within 3D matrices

Fig. 6

Representative maximum projection fluorescence images of NPC-derived neurons within Dynamic Fast (a) and Dynamic Slow (b) HELP gels after 14 days of differentiation labeled with the synaptic markers PSD95 (green) and SYN1 (white). c Time-lapse microscopy of four representative NPC-derived neurons within Dynamic Fast HELP gels after 14 days of differentiation incubated with the calcium-sensitive Fluo-4AM dye and treated with γ-aminobutyric acid (GABA) (left). Quantified relative fluorescence of Fluo-4AM for cells 1-4 over time (right). Source data are provided as a Source Data file.

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