Fig. 1: Schematic of different scanning patterns.
From: Reduction of SEM charging artefacts in native cryogenic biological samples
A, B Field of view (black rectangle) imaged using a 7 × 7 array where the rainbow colour corresponds to the temporal distribution of the fluence (red at the start, blue at the end of the time sequence). A Raster scanning. B Interleaved scanning which skips 2 positions in x and 2 positions in y. C–F Overlay of vitrified RPE-1 with a schematic representation of pixel positions (black squares) in a 16 × 16 array. The green cone represents the electron beam. The straight arrows represent raster scanning while the curved arrows indicate pixel skipping during interleaved scanning. The colour of the arrow represents the pixel acquired (white) or yet to be acquired (yellow). E, F Spatial distribution of the electron fluence and isotropic charge dissipation (red). Yellow arrows indicate pixels have been imaged; green arrows indicate pixels yet to be imaged. Red circles illustrate charge dissipation radiating outwards at each position.
