Fig. 3: Charging artefact reduction in RPE-1 cells allows the observation of lipid droplet (LD) surrounding environments.
From: Reduction of SEM charging artefacts in native cryogenic biological samples
Vitrified cells imaged at 52° with respect to the FIB milled sample plane using a 100 ns dwell time x100 repetitions. A–C Overview of a slice from RPE-1 imaged using raster line integration, raster frame integration and interleaved frame integration, respectively. Scale bar: 2 µm. D–F enlargement of the pink boxes in (A–C). White arrow: membrane in the vicinity of a LD. Black arrow: content of a degradative compartment. m: mitochondria. Scale bar 0.5 µm. G–I Enlargement of the green boxes in (A–C); brightness and contrast were modified to improve visualisation. White arrow: wrapping of endoplasmic reticulum (ER) around LD. Scale bar 1 µm.
