Fig. 5: SEM volume imaging of mouse brain.
From: Reduction of SEM charging artefacts in native cryogenic biological samples
A 118-day old mouse brain imaged at 90° with respect to the FIB milled sample plane using 100 ns dwell time ×100 repetitions. A 1334 µm³ volume in focus was aligned and manually segmented for the region of interest. A Segmented volumes. B, C Slices from the volume with overlayed segmentation. Scale bar 2 µm. Boxes in A are superimposed on B and C and enlarged in boxes (D–G) with the respective coloured box containing representative slices in the volume. Numbers indicate the z-location within the volume. D thin myelin axon with internal cytoplasmic channels (CC). Scale bar: 250 nm. E intermediate axon with extended CC, mitochondria (m). Scale bar: 250 nm. F Thick myelin, large axon and the interaction with 2 oligodendrocytes showing the inner tongues (IT) and the outer tongues (OT). Scale bar: 500 nm. Mitochondria are also visible in the axon and one of the outer tongues. G Microglia cells and their proximity to different axons showing a stretch of colocalization, as well as internal organelles including mitochondria (m), vesicles (v) and inner tongues (IT). Scale bar 500 nm.
