Fig. 2: Loss of TANGO2 affects cytoskeletal and membrane structure, protein transport and glycosylation.

a Transcriptome-wide changes in TANGO2^–/– cells compared with controls (n = 3). Increased genes are shown in red and decreased genes in blue. b GO analysis based on transcriptomic changes showing significantly changing pathways involved in biological processes, molecular function and cellular component in TANGO2^–/– cells compared with control cells. c Protein changes in TANGO2^–/– cells compared with control cells identified by mass spectrometry (n = 5 per genotype), the inset shows the number of significantly increased (red) or decreased (blue) proteins and adjusted p values are shown in Supplementary Data 2. Significantly increased and decreased proteins are shown in light red and light blue, respectively, mitochondrial proteins are shown in green, Golgi proteins are shown in purple and intermediate filament proteins are shown in yellow. d GO analyses based on proteomic changes show significantly changing reactome pathways in TANGO2^–/– cells compared with control cells. The results show the top 20 pathways with the highest –log₁₀(FDR) and the colour scale represents fold change (FC) for each pathway and set size is the number of genes within each pathway. e N-glycomic profile of control and TANGO2^–/– cells. f Changes in glycosylating enzymes identified by RNA-seq. The colour scale represents fold change (FC). All values shown in panels (e and f) are means ± SD *p < 0.05, **p < 0.01 ***p < 0.001, ****p < 0.0001, Student’s two-tailed t test (n = 5 biological replicates).