Fig. 3: Attachment of bacterial pathogens to flat, pro- (Topo-Units 697 and 336) and anti-attachment (881 and 685) PS. | Nature Communications

Fig. 3: Attachment of bacterial pathogens to flat, pro- (Topo-Units 697 and 336) and anti-attachment (881 and 685) PS.

From: Combinatorial discovery of microtopographical landscapes that resist biofilm formation through quorum sensing mediated autolubrication

Fig. 3

a Representative images of P. aeruginosa, S. aureus, Pr. mirabilis and A. baumannii attachment (bright field images of the TopoUnits shown in top row) after 4 h incubation under static conditions. Scale bar: 50 µm. b Quantification of normalised mean fluorescence intensity of bacterial cells stained with Syto9 and grown on the same topographies (P. aeruginosa n = 22 TopoUnits, S. aureus n = 6 TopoUnits, Pr. mirabilis n = 12 TopoUnits and A. baumannii n = 16 TopoUnits). Data shown in boxes extend from the 25th to 75th percentiles and lines in boxes correspond to the median values. Whiskers go down to the smallest and up to the largest values. Statistical analysis was done using a two-way ANOVA with Dunnett’s multiple comparisons test (*p < 0.05; **p < 0.01; ***p < 0.001; **** p < 0.0001). P-values for 881 and 685 topographies are: P. aeruginosa = 1.28E−12 and 7.05E−13, S. aureus = 0.00032 and 0.0011, Pr. mirabilis = 9.25E−07 and 3.22E−07, A. baumannii = 2.72E−09 and 6.37E−11. The source data are provided as a Source Data file.

Back to article page