Fig. 4: CpE@BMV scavenges the excessive ROS of LPS-induced macrophages. | Nature Communications

Fig. 4: CpE@BMV scavenges the excessive ROS of LPS-induced macrophages.

From: Bacterial membrane nanovesicles encapsulating prodrug assemblies combine chemical and immunological therapies for chronic bacterial infection

Fig. 4

a–e ROS scavenging efficiency of CpE@BMV toward H2O2 (a), •OH (b), •O2– (c), ABTS+ (d), and DPPH (e) at different nanoparticles concentrations (n = 3 independent samples). f Fluorescence images of intracellular ROS in LPS-treated RAW264.7 cells after various treatments. RAW264.7 cells were stained with a DCFH-DA fluorescence probe. Scale bar: 20 μm. g–j The levels of TNF-α (g), IL-6 (h), IL-1β (i), and IL-10 (j) in the supernatant of RAW264.7 macrophages after different treatments for 24 h were examined using ELISAs. Data are presented as means ± s.d. (n = 3 biologically independent samples). Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test, p > 0.05, no significance (ns), *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001.

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