Fig. 6: Transcriptome sequencing and microbiota analysis in the pulmonary model on day 7 post various treatments. | Nature Communications

Fig. 6: Transcriptome sequencing and microbiota analysis in the pulmonary model on day 7 post various treatments.

From: Bacterial membrane nanovesicles encapsulating prodrug assemblies combine chemical and immunological therapies for chronic bacterial infection

Fig. 6

a Volcano plot displayed the distributions of upregulated and downregulated genes (≥2-fold difference, p-value < 0.05) in the CpE@BMV group compared to the PBS group. None, non-differentially expressed genes (DEGs). b Heatmaps of different groups of upregulated and downregulated DEGs related to immune response pathways. c, d Gene ontology (GO) enrichment analysis of DEGs performed for both upregulated (c) and downregulated (d) GO terms between the CpE@BMV-treated mice and PBS-treated mice. e, f Gene set enrichment analysis (GSEA) of DEGs enriched in PPAR signaling pathway (e) and cellular response to VEGF stimulus (f). g Relative abundance of the top 7 family-level taxa in the lung microbiome across healthy mice, PBS, Cip, and CpE@BMV treatments. h Relative abundance of various taxa in the healthy mice, PBS, Cip, and CpE@BMV treatments. Red, pathogenic strains in lung infection. Green, non-pathogenic strains. i Predicted functional compositions of the microbial communities in the healthy mice, PBS, Cip, and CpE@BMV treatments. Data are presented as means ± s.d. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test, p > 0.05, no significance (ns), *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001.

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