Fig. 1: Reduction in pro-inflammatory bioactive lipid biosynthesizing enzymes during myogenic progression.
From: Bioactive lipid mediator class switching regulates myogenic cell progression and muscle regeneration
a Graphical overview of the myoblast isolation, culture, and differentiation experiment (Created in BioRender)88. b Graphical representation of the leukotriene synthesis cascade (Created in BioRender)89. c–h Expression of leukotriene synthesis enzymes quantified by qPCR: Alox5 (c) (P vs. D1 p < 0.0001; P vs. D2 p < 0.0001; P vs. D3 p < 0.0001; P vs. D4 p < 0.0001; P vs. D5 p < 0.0001), Alox5ap (e) (P vs. D1 p < 0.0001; P vs. D2 p < 0.0001; P vs. D3 p = 0.0002; P vs. D4 p = 0.0012; P vs. D5 p < 0.0009), Lta4h (f) (P vs. D2 p = 0.0133; P vs. D3 p = 0.0023; P vs. D4 p = 0.0026; P vs. D5 p = 0.0009), Ltc4s (g) (P vs. D1 p < 0.0001; P vs. D2 p < 0.0001; P vs. D3 p < 0.0001; P vs. D4 p < 0.0001; P vs. D5 p < 0.0001), Mgst2 (h) (P vs. D1 p = 0.0124; P vs. D2 p = 0.0116; P vs. D3 p = 0.0038; P vs. D4 p = 0.0033; P vs. D5 p = 0.0022). d, k Representative Western Blots and quantification of the enzymes ALOX5 (d) (P vs. D1 p = 0.0045; P vs. D2 p = 0.0003; P vs. D3 p = 0.0001; P vs. D4 p = 0.0003; P vs. D5 p < 0.0001) and COX-2 (k) (P vs. D1 p = 0.0073; P vs. D2 p = 0.0013; P vs. D3 p = 0.0003; P vs. D4 p = 0.0001; P vs. D5 p = 0.0006) expression during myogenesis (relative to GAPDH or β-actin as loading control). i Graphical representation of the prostaglandin synthesis cascade (Created in BioRender)89. j, l–o Expression of prostaglandin synthesis enzymes quantified by qPCR: Ptgs2 (j) (P vs. D1 p < 0.0001; P vs. D2 p < 0.0001; P vs. D3 p < 0.0001; P vs. D4 p < 0.0001; P vs. D5 p < 0.0001), Tbxas1 (l), Ptges2 (m), Ptgis (n), Ptgds (o) (P vs. D1 p = 0.0061; P vs. D2 p = 0.0006; P vs. D3 p = 0.0050; P vs. D4 p = 0.0489). Results are expressed as mean +/- SEM. Biological replicates n = 5 for panel c; n = 4 for panels d, e, g, h, m, n; n = 6 for panel j; n = 3 for panels f, k, l, o. *p < 0.05, **p < 0.01, *** p < 0.001. One-Way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source Data file.
