Fig. 2: Increase in pro-resolving lipid biosynthesizing enzymes during myogenic progression.
From: Bioactive lipid mediator class switching regulates myogenic cell progression and muscle regeneration
a Graphical representation of the pro-resolving lipid mediator cascade (Created in BioRender)90. b, c, e Expression of pro-resolving enzymes quantified by qPCR: Pla2g2d (b) (P vs. D3 p = 0.0413; P vs. D4 p = 0.0088; P vs. D5 p = 0.0218), Alox15 (c) (P vs. D3 p = 0.0487; P vs. D4 p = 0.0057) and Alox12 (e) (P vs. D3 p = 0.0169). d Representative Western Blots and quantification of the enzyme ALOX15 during myogenesis (relative to β-actin as loading control) (P vs. D5 p = 0.0133). f Expression of the prostaglandin-degrading enzyme Hpgd quantified by qPCR (P vs. D4 p = 0.0430; P vs. D5 p = 0.0002). g Expression of Ptgs2 after 24 h treatment with prostaglandins PGE2 (250 nM) or vehicle (p = 0.0221). h Expression of Hpgd after 24 h treatment with prostaglandins PGE2 (250 nM), PGD2 (250 nM) and 15Δ-PGJ2 (250 nM) (Veh vs PGE2 p = 0.0103; Veh vs 15Δ-PGJ2 p = 0.0155). i Expression of Ptgs2, Ptges, Hpgd, Ptgds, and Myog from single cell RNA sequencing dataset (Oprescu SN et al. iScience 2020) from uninjured, 0.5, 2, 3.5, 5, 10, and 21 days post-injury (dpi) in WT muscle. j Proportion of cells expressing Ptgds in MuSCs (Pax7+MyoD-), myoblasts (Pax7-MyoD + ), or differentiated myoblasts (Pax7-Myog + ) in WT muscle at 3.5 dpi (Oprescu SN et al. iScience 2020). Results are expressed as mean +/- SEM. Biological replicates n = 3 for panels b, d, f; n = 6 for panels c, e; n = 4 for panel g; n = 5 for panel h (except n = 3 for PGD2 and 15Δ-PGJ2 groups). *p < 0.05, **p < 0.01, *** p < 0.001. 2-way Paired t-test (g, h), One-way ANOVA with Dunnett’s multiple comparisons test (b, c, d, e, f). Source data are provided as a Source Data file.
