Fig. 3: Targeted lipidomics identifies the bioactive lipid class switching from pro-inflammatory lipids to pro-resolving lipids during myogenic progression.
From: Bioactive lipid mediator class switching regulates myogenic cell progression and muscle regeneration
a Graphical representation of the pro-inflammatory and pro-resolving lipid mediator cascade (green). Bioactive lipid receptors are identified in gray boxes. (Created in BioRender)91. b–e, j–m Quantification by mass spectrometry (LC-MS/MS) of lipid mediator levels measured in the medium of proliferating vs differentiating myoblasts (day 1, 3, 5): PGF2a (b) (P vs. D1 p = 0.0134; P vs. D3 p = 0.0329), PGE2 (c) (P vs. D1 p = 0.0134; P vs. D3 p = 0.0329), TXB2 (d) (P vs. D1 p = 0.0001; P vs. D3 p = 0.0007; P vs. D5 p = 0.0009), PGD2 (e), 15(R)-LXA4 (j) (P vs. D1 p = 0.0076; P vs. D3 p = 0.0027; P vs. D5 p = 0.0065), RvE1 (k), RvD5n-3 DPA (l) (P vs. D1 p = 0.0096), PDX (m). f–i,n–q Quantification by qPCR of bioactive lipid receptors’ expression in myogenic cells during myogenesis in vitro: Ptgfr (f) (P vs. D1 p < 0.0001; P vs. D3 p < 0.0001; P vs. D5 p < 0.0001), EP4 (g) (P vs. D1 p < 0.0001; P vs. D3 p < 0.0001; P vs. D5 p < 0.0001), Tbxa2r (h), DP2 (i) (P vs. D5 p = 0.0176), Fpr2 (n) (P vs. D1 p < 0.0001; P vs. D3 p = 0.0003; P vs. D5 p < 0.0001), Cmklr1 (o), Gpr101 (p) (P vs. D5 p = 0.0150), Gpr37 (q) (P vs. D5 p = 0.0068). a–q Pro-inflammatory lipids and receptors are identified in red, pro-resolving lipids and receptors are identified in green, and PGD2/DP2 that play a role in triggering the switch are identified in orange. Results are expressed as mean +/- SEM. Biological replicates n = 4 for panels b–d, g, j, l, n–q; n = 3 for panels e, f, h, i, k, m. *p < 0.05, **p < 0.01, *** p < 0.001. Repeated measure one-way ANOVA with Dunnett’s multiple comparisons test (b–e, j–m), One-way ANOVA with Dunnett’s multiple comparisons test (f–i, n–q). Source data are provided as a Source Data file.
