Fig. 6: Alox15-KO mice display impaired regeneration.

a Schematic showing the timeline of cardiotoxin (CTX) injury experiment and muscle collection at 7- and 14-days post injury (dpi) (Created in BioRender)⁹². b Representative images of Pax7 (red), DAPI (blue) staining on muscle sections of tibialis anterior (TA) muscle of uninjured WT and Alox15-KO mice. Scale bars = 50 μm. c Quantification of Pax7+ cells in TA muscles of uninjured WT and Alox15-KO mice. d Representative images of H&E staining on muscle sections of TA muscle of uninjured WT and Alox15-KO mice. Scale bars = 50 μm. e Quantification of the minimal fiber diameter (MFD) in TA muscles of uninjured WT and Alox15-KO mice. f Representative images of Pax7 (red), Myog (green), DAPI (blue) staining on muscle sections of TA muscle of WT and Alox15-KO mice at 7 dpi. Scale bars = 50 μm. g, h Quantification of the number of Pax7+ (g) (p = 0.0287) and Myog+ (h) (p = 0.0022) cells in TA muscles of WT and Alox15-KO mice at 7 dpi. i Representative images of H&E staining on muscle sections of TA muscle of WT and Alox15-KO mice at 7 dpi. Scale bars = 50 μm. j Quantification of the minimal fiber diameter in TA muscles of WT and Alox15-KO mice at 7 dpi (p = 0.0377). k Representative images of Pax7 (red), Myog (green), DAPI (blue) staining on muscle sections of the TA muscle of WT and Alox15-KO mice at 14 dpi. Scale bars = 50 μm. l, m Quantification of the number of Pax7+ (l) and Myog+ (m) (p = 0.0143) cells in the TA muscles of WT and Alox15-KO mice at 14 dpi. n Representative images of H&E staining on muscle sections of the TA muscles of WT and Alox15-KO mice at 14 dpi. Scale bars = 50 μm. o Quantification of the minimal fiber diameter in the TA muscles of WT and Alox15-KO mice at 14 dpi (p = 0.0355). p Schematic showing the timeline of mdx mice CTX-injury experiment followed by the transplantation of WT and Alox15-KO myoblasts in the right and left TA muscles at 3 DPI and muscle collection at 14 dpi (Created in BioRender)⁹². q Representative images of Dystrophin (pink), WGA (green), DAPI (blue) staining on muscle sections of TA muscle of mdx mice at 14 dpi. Scale bars = 75 μm. r Quantification of Dystrophin+ myofibers in TA muscles of mdx mice at 14 dpi transplanted with WT or Alox15-KO myoblasts (p = 0.0336). Results are expressed as mean +/- SEM. Black bars represent the WT group and red bars are the Alox15-KO group. Biological replicates n = 5 for panel (c); n = 3 for panel (e); n = 3 for WT and n = 6 for Alox15KO for panels (g, h, l, m); n = 4 for panels (j, r); n = 3 for WT and n = 7 for Alox15KO for panel (o). * p < 0.05, **p < 0.01, *** p < 0.001. 2-way Unpaired t-test. Source data are provided as a Source Data file.