Fig. 8: Protectin D1 promotes muscle regeneration in mdx mice.
From: Bioactive lipid mediator class switching regulates myogenic cell progression and muscle regeneration
a Schematic showing the timeline of daily intraperitoneal (i.p.) injection of Protectin-D1 (PD1, 5 ug/kg/day), or vehicle (Veh) in mdx mice for 21 days (Created in BioRender)94. b Representative micrographs of Pax7 (red), Myog (green), and DAPI (nuclei, blue) staining on sections of the tibialis anterior (TA) muscles of mdx mice treated with PD1 or vehicle. Scale bars = 50 μm. c, d Quantification of the number of Pax7+ (c) (p = 0.0137), and Myog+ (d) (p = 0.0303) cells in muscles of mdx mice after 21 days of PD1 or Veh treatment. e Representative micrographs of Laminin (green), DAPI (nuclei, blue) staining on muscle sections of the TA muscle of mdx mice after 21 days of PD1 or Veh treatment. Scale bars = 50 μm. f, g Quantification of the cross-sectional area (f) (p = 0.0414) and number of myonuclei per fiber (g) (p = 0.0376) in the TA muscles of mdx mice after 21 days of PD1 or Veh treatment. h Isometric contractile properties of EDL muscles showing the specific force of mdx mice treated daily for 21 days with PD1 or Veh (80hz p < 0.0017; 100hz; p < 0.0001; 150hz; p = 0.0005). Results are expressed as mean +/- SEM. Black bars represent the mdx group and blue bars are mdx + PD1 group. Biological replicates n = 4 for panel c, n = 6 for panel d; n = 5 for mdx and n = 6 for mdx + PD1 for panels (f, g); n = 5 for panel (h). *p < 0.05, **p < 0.01, *** p < 0.001. 2-way Unpaired t-test (c, d, f, g), 2-way ANOVA with Uncorrected Fisher’s LSD multiple comparisons test (h). Source data are provided as a Source Data file.
