Fig. 3: Whisker motion, body motion, and arousal do not account for whisker-specific behavioral effects.
From: Reward history guides focal attention in whisker somatosensory cortex
A Mean whisker motion, platform motion (proxy for body motion), and pupil size traces, from DeepLabCut analysis across 43,647 trials, 74 sessions in 9 mice. Each panel shows the last 3 s of the intertrial interval (ITI) period after the prior trial, plus the current trial stimulus period. Traces and shading are mean ± SEM across all trials. Dashed line, stimulus onset (Go trials) or dummy piezo onset (NoGo trials). Pupil size was normalized within each session to the mean pupil size across the whole session. Bottom row, lick histogram. B Mean whisker movement, body movement, and pupil size change during the stimulus period. Prior Hits increased stimulus-evoked whisker and body motion on subsequent trials (Δ whisker motion, prior > 1 hit same vs prior NoGo: p = 1e-4, prior > 1 hit same vs > 1 hit different: p = 0.39; Δ body motion, prior > 1 hit same vs prior NoGo: p = 1e-4, prior > 1 hit same vs > 1 hit different: p = 0.39; Δ pupil area, prior > 1 hit same vs prior NoGo: p = 0.49, prior > 1 hit same vs > 1 hit different: p = 0.53). p-values are for > 1 Prior Hit vs Prior NoGo (top), and > 1 Prior Hit Same vs > 1 Prior Hit Different (right) (permutation test). Error bars show SEM across trials. C Design of Botox experiment. Behavior was assayed on an average of 12 sessions pre-Botox injection, and 7 sessions post-Botox whisker paralysis. D Reward history-dependent attention effect in each of the 4 mice tested, for standard sessions (before Botox, open symbols) and Botox sessions (filled symbols). M, Mouse numbers as in Fig. 1J. Large points are mean ± SEM across mice. Conventions as in Fig. 1J. Whisker paralysis did not alter the mean whisker-specific d-prime effect or criterion effect (p = 0.88, permutation test comparing same vs. different shifts in Δd′ for standard and Botox sessions. Paired differences in same vs. different Δd′ shifts across session types were tested against zero). See also Supplementary Fig. S2.
