Fig. 3: Endogenous glycolytic enzymes are also enriched in LifeAct labelled waves.
From: Self-organizing glycolytic waves tune cellular metabolic states and fuel cancer progression
a Time-lapse confocal images showing the basal surface of MCF-10A-M3 cells expressing LifeAct-RFP (shown in Supplementary Fig. 12) and PFK-GFP before and after fixation by 4% PFA (added at time 0). Yellow arrow heads indicate examples of waves. Time stamp is minute. The decrease in fluorescence intensity was due to partial dissipation of GFP-tagged proteins caused by cell permeabilization. Fluorescence signals after fixation were multiplied by 2 for better presentation. This shown image represents a typical example of cells from N ≥ 3 independent experiments. b–k Confocal images showing the basal surface of 4% PFA fixed MCF-10A-M3 cells expressing LifeAct-RFP and stained with antibodies against GFP (b), GAPDH (c, d), Aldolase (e, f), Enolase 1 (g, h), PFK (i, j), and HK-1 (k). Channels of antibody staining, LifeAct, and merged are shown. The units of the scale bar are indicated in each image. Each image shown in (b-k) represents a typical example of cells from N ≥ 3 independent experiments.
