Fig. 1: Establishment of an ex vivo uterine system.

A Isolation of endometrial fragments from dpc 3.75 pregnant recipient mice and E3.75 blastocysts from donor mice. B A stereomicroscopic view of Isolated endometrium and endometrial fragments. Scale bar: 1 mm. C Specification of a PDMS ceiling. D A photograph of the co-culture of embryos and an endometrial fragment on agarose gel covered with a PDMS ceiling. White arrow, an endometrial fragment. E Schematic illustration of ex vivo implantation system. A, C, and E. created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en.