Fig. 3: Ex vivo uterus harbors genuine cellular components during culture.

A–C The UMAP of single-cell RNA-seq of the whole endometria (A) showed similar cell clustering between in vivo and ex vivo. Epithelial cells and stromal cells were further profiled in B, C. Epi, epithelium; Str, stroma; VE, vascular endothelium; VSM, vascular smooth muscle; Mf, macrophages; ILC, innate lymphoid cells; LE_attached, luminal epithelium at embryo-attached sites; LE_unattached, luminal epithelium at embryo-unattached sites; LE_inflam, inflammatory luminal epithelium; GE, glandular epithelium; GE_inflam, inflammatory glandular epithelium; Attached, the stroma of embryo- or PDMS-attached sites (attached stroma); Non_prolif, non-proliferating stroma; Prolif, proliferating stroma; Dec, decidualized stroma; Sub_epi, subepithelial stroma; Sub_VE, subvascular endothelial stroma; Angio, pro-angiogenic fibroblasts; Hypoxy, hypoxic fibroblasts; Inflam, inflammatory fibroblasts.