Fig. 4: Changes in membrane biogenesis, motility and adhesion across the cell cycle.

A Major steps of membrane biogenesis in S. islandicus. Dashed line represents possible modifications the polar head may have. IPP, isopentenyl pyrophosphate; DMAP, dimethylallyl monophosphate; GGPP, geranylgeranyl diphosphate; GGGP, geranylgeranylglyceryl phosphate; G1P, glycerol-1-phosphate; DGGGP, digeranylgeranylglyceryl phosphate; Tes, tetraether synthase; Cds, calditol synthase. B Expression of membrane biogenesis enzymes. Box plot of the expression of the enzymes in the 15 replicates. The center line represents the median; the box limits, the first and third quartiles; whiskers extend 1.5 times the interquartile range; data beyond the whiskers are outliers represented as points. Statistical significance is extracted from the DGE analysis, calculated with the limma package and p values were adjusted for multiple comparisons using the Benjamini-Hochberg method. * = p value ≤ 0.01. ** = p value ≤ 0.001. *** = p value ≤ 0.0001. Exact p values are in Supplementary Data 1. C Gene co-expression subnetworks of membrane biogenesis enzymes. Subnetworks contain only those nodes that co-express with at least two membrane biogenesis enzymes. Nodes are colored by arCOG category as in Fig. 2B, positive edges are green and negative edges, red. C inset. Line plot representing the number of nodes of the subnetwork of all genes co-expressing with the membrane biogenesis enzymes. D Expression of adhesive pili and archaellum. Box plot of their expression in the 15 replicates as in (B). Statistical significance as in (B). E Gene co-expression subnetwork at the S phase of the adhesive pili and archaellum. The subnetwork contains only those nodes that co-express with at least two of their components. Coloring as in (C). E inset. Line plots represent the number of nodes of the subnetworks of all genes co-expressing with the adhesive pili and the archaellum.