Fig. 6: IP injections of ART5803 reverse GluN1 reduction induced by pathogenic autoantibody (#003-102 Ab) in marmoset disease model.
From: Monoclonal humanized monovalent antibody blocking therapy for anti-NMDA receptor encephalitis
a Representative immunostaining patterns of human IgG, with anti-human IgG, 3,3’-diaminobenzidine (DAB) staining as brown, and nuclei hematoxylin staining as blue, in sagittal brain sections of marmosets without treatment (treatment naïve control) or treated with either #003-102 Ab ICV infusion at 10 µg/h for 4 weeks (#003-102 Ab ICV) or ART5803 IP injections at 400 mg/kg twice a week for 2 weeks (ART5803 IP). The treatment naïve control marmoset brain serves as negative background control. Slides were scanned at 40x magnification. The scale bar is 5 mm. FC = frontal cortex. b Comparison of GluN1 (NMDAR) and GluR2 (AMPA receptor) levels by immunostaining in the frontal cortex of marmosets without treatment (treatment naïve control, n = 3) or treated with #003-102 Ab ICV plus vehicle IP injections (n = 7) or ART5803 IP injections (n = 8), quantified using DAB positive superpixel segmentation in QuPath. Data are represented as mean ± SEM. Statistical analysis was performed using unpaired, two-tailed, Student’s t-tests. *p < 0.05; ns = not significant. Representative heat maps demonstrating DAB intensity, with black indicating low signal, and yellow, high signal. Scale bars are 5 mm. Source data are provided as a Source Data file. c Comparison of GluN1 (NMDAR), GluR2 (AMPA receptor), PSD95 (synapse marker) and GAPDH levels by western blot analysis in the cerebral cortex (whole) of marmosets without treatment (treatment naïve control, n = 3) or treated with #003-102 Ab ICV plus vehicle IP injections (n = 7) or ART5803 IP injections (n = 8). Quantitative comparisons between samples on different blots were normalized to the treatment naive control for total protein. All samples were from the same experiment and blots were processed in parallel. Data are represented as mean ± SEM. Statistical analysis was performed using two-tailed, unpaired Student’s t-tests. *p < 0.05; ns = not significant. Uncropped blots are reported in Supplementary Fig. S10.
