Fig. 2: Calcium imaging of A27h central processes.
From: Closed-loop two-photon functional imaging in a freely moving animal
2nd instar larva expressed GCaMP6f and hexameric mCherry under R36G02 control. During the 253 s recording shown, the larva traveled 5.3 mm from its original position along a path length of 1 cm (a) X-Y and X-Z projection of template image created from the mCherry channel of labeled VNC. Colored circles indicate VOIs analyzed in (b, d, e, h, i). VOIs progress numerically from posterior to anterior. The white dashed line indicates the volume projected in (c, f, g). b Ratiometric (GCaMP6f/mCherry) measurement of activity in VOIs over the time course of the experiment, normalized separately for each VOI. Periods of forward (teal box labeled with F) and backward (red hatched box labeled with B) crawling are indicated below. Unlabeled periods represent turns and pauses. c Time-space projection of ratiometric activity measure. The vertical (space) axis spans the range from the bottom of the white box to the top. The horizontal (time) axis matches (b). The color scale is normalized so that the median of the entire data set shown in the panel is 1. Expanded views of VOI ratio (d, e) and projected ratios (f, g) during forward (d, f) and backward (e, g) crawling. c, f, g use the same color scale. Cycle-average ratiometric activity measure in each VOI, for forward (h) and backward (i) crawling vs. cycle phase (ϕ). Each VOI is normalized separately so that the minimum mean activity during forward crawling is 1.
