Fig. 4: Atg44 is dispensable for the Ringo morphology formation.

a Dextrose and glycerol serial dilutions of WT, dnm1Δ and dnm1Δ+DNM1 strains at 23, 30 and 37 °C. b SIM acquisitions (z-projections) of dnm1Δ+DNM1 cells labeled with mt-mCherry and Tom70-GFP in fermentation (top) or respiration (bottom). Scale bar, 2 µm. Right graph: percentage of cells with Tubular, Ringo, Fragmented, Aggregated, Hyperfused or HFR mitochondria during fermentation (SD, orange) or respiration (SG, blue). Mean ± s.d. from >64 cells in n = 3 independent experiments (colored circles). ***p = 0.0004, ****p < 0.0001 (Two-way Anova test followed by Tukey’s multiple comparisons test) (c) Same as (b) with WT, dnm1Δ or atg44Δ cells in YPD (top) or YPG (bottom) media. d Average mitochondrial thickness per cell from (c), in YPD or YPG as quantified in SIM. Violin plots from n = 74 to 75 cells with mean indicated at the bottom and by black dashed lines (quartiles with dotted lines). ****p < 0,0001,ns p = 0.9966, **p = 0.0089, ****p < 0.0001 (Two-way Anova test followed by Tukey’s multiple comparisons test) (e) Percentage of WT and atg44Δ cells from (c) with Tubular, Ringo, Fragmented, Aggregated, Hyperfused, HFR or Ringo-like mitochondria during fermentation (YPD, orange) or respiration (YPG, blue). Mean ± s.d. from > 73 cells in n = 3 independent experiments (colored circles). f Same as (a) with WT, dnm1Δ, atg44Δ, mdv1Δ, fis1Δ and caf4Δ cells at 37 °C (see also Supplementary Fig. 8d). For all graphs, ns, not significant.