Fig. 5: Autophagy is reduced in Bthal LT-HSCs. | Nature Communications

Fig. 5: Autophagy is reduced in Bthal LT-HSCs.

From: Imbalanced TGFβ signalling and autophagy drive erythroid priming of hematopoietic stem cells in β-thalassemia

Fig. 5

A DEGs carrying BMP responsive element (BMP-RE) between Bthal and HD HSC/MPP. Gene Ontology (GO) analysis of 120 target genes of BMP pathways, padj <0.01. Terms with adjusted p-value (Benjamini-Hochber correction). B GSEA of categories from MSigDB database in HSC/MPP pool of Bthal samples compared to HD, FDR < 0.05. C Genes of autophagy deregulated in Bthal HSC. Normalized UMI counts of selected genes, involved in Autophagy, differentially expressed between Bthal HSCs (red) and HD HSCs (white). Results are represented as Fold respect to Ctrl (HD n = 2; Bthal n = 2). D Representative microscopy images of DAPI (blue) and LC3 staining (green) of purified HD and Bthal sorted LT-HSCs. Scale bar 10 μm. n = 3 independent experiments (n = 3 HD samples and n = 3 Bthal samples). E Quantification of LC3 median intensity of evaluated foci in HD and Bthal LT-HSCs (HD n = 3; Bthal n = 3). About total 100 nuclei were analyzed for condition. P-values by two-tailed unpaired t-test; mean ± SEM. F the area of evaluated foci in HD and Bthal LT-HSCs (46 nuclei from HD n = 3; 32 nuclei from Bthal n = 3). P-values by two-tailed unpaired t-test; mean ± SEM. Source data are provided as Source Data file.

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