Fig. 1: TUG localizes to the ERGIC.
From: TUG protein acts through a disordered region to organize the early secretory pathway
a–d Confocal images are shown of TUG KO HeLa cells co-transfected with mCherry-tagged TUG (Tug-mCherry; magenta) together with Emerald-tagged ERGIC53 (Em-ERGIC53; a; green), the ERES marker Sec23-GFP (b; green), the Golgi marker mNG-tagged GMAP210 (GMAP210-mNG; c; green) and Stx12-GFP (d; green). Note the colocalization between TUG and ERGIC53 in peripheral punctate structures. TUG puncta also are positive for Stx12 (yellow circles), but are distinct from the ERES (Sec23) and are excluded from the Golgi apparatus (GMAP210). Images for co-transfection of Tug-mCherry with Em-ERGIC53 are representative of three independent experiments with similar results. Those for Sec23-GFP and GMAP210-mNG and Stx12-GFP are representative of two independent experiments with similar results.
