Fig. 4: The reconstituted ureters mature to the postnatal stage.
From: In vitro generation of a ureteral organoid from pluripotent stem cells
a UMAP plots of in vivo mouse kidneys with ureters (E17.5, P0, P7) and the reconstituted ureter combined mouse embryonic ureteral epithelia and mouse ESC-derived ventral iSPs. The clusters of the ureteral mesenchyme and the urothelium are indicated by red (cluster 30) and black (cluster 13) arrows, respectively. b Unbiased hierarchical clustering analysis of the ureteral mesenchyme (cluster 30 in Fig. 4a) of in vivo ureters (E17.5, P0, P7) and the reconstituted ureter (rUreter). c Unbiased hierarchical clustering analysis of the urothelium (cluster 13 in Fig. 4a) of in vivo ureters (E17.5, P0, P7) and the reconstituted ureter (rUreter). d Dot plots of genes related to mesenchymal-epithelial interactions during ureteral development in the ureteral mesenchyme (cluster 30 in Fig. 4a). e Dot plots of genes related to mesenchymal-epithelial interactions during ureteral development in the urothelium (cluster 13 in Fig. 4a). Pod podocytes, PT proximal tubule, LoH loop of Henle, DT distal tubule, UE ureteral epithelium, Ren renin cells, Mes mesangial cells, oMS outer medullary stroma, iMS inner medullary stroma, UM ureteral mesenchyme, CD collecting duct, NP nephron progenitor, SP stromal progenitor, UB ureteric bud, EC endothelial cell.
